Phosphoribosylpyrophosphate and enzymes of purine metabolism in erythrocytes from young hyperuricemic males |
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| Authors: | A G van Maris W J Tax T L Oei C H de Bruyn F Klein S J Geerts J H Veerkamp H A Valkenburg |
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| Affiliation: | 1. Department of Human Genetics, Faculty of Medicine, University of Nijmegen, Nijmegen, The Netherlands;2. Department of Biochemistry, Faculty of Medicine, University of Nijmegen, Nijmegen, The Netherlands;3. Department of Epidemiology, Erasmus University, Rotterdam, The Netherlands;1. State key laboratory of fluid power and mechatronic systems, Zhejiang University, Zheda Road 38, 310027, Hangzhou, China;2. Department of Mechanical and Industrial Engineering, 3283 Patrick Taylor Hall, Louisiana State University, Baton Rouge, LA, United States;1. Department of Biochemistry, Medical University of Gdansk, 1 Debinki St., 80-211 Gdansk, Poland;2. Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, 48 Wojska Polskiego St., 60-627 Poznan, Poland;3. Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 5a Pawinskiego St., 02-106 Warsaw, Poland;4. Heart Science Centre, Imperial College at Harefield Hospital, Middlesex UB9 6JH, Harefield, UK;1. Department of Medical Biochemistry, Amsterdam Cardiovascular Sciences, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, the Netherlands;2. Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA;3. Oncology Department, Institut de Cancérologie Gustave Roussy, Villejuif, France;4. Radiology Department, Institut de Cancérologie Gustave Roussy, Paris, France;5. Biomedical MR Imaging and Spectroscopy Group, Center for Image Sciences, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands;6. Laboratory of Chemical Biology, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands;7. Faculty of Medicine, Universite Paris-Saclay, Le Kremlin-Bicêtre, France;8. Department of Hematology, Amsterdam University Medical Centers, Vrije Universiteit Amsterdam, Amsterdam, the Netherlands;9. Institute for Cardiovascular Prevention, Ludwig Maximilian University of Munich, Munich, Germany;10. German Centre for Cardiovascular Research, partner site Munich Heart Alliance, Munich, Germany;1. The Pirbright Institute, Ash Road, Pirbright, Surrey, GU24 0NF, United Kingdom;2. The Met Office, FitzRoy Road, Exeter, Devon, EX1 3PB, United Kingdom |
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| Abstract: | Red blood cell phosphoribosylpyrophosphate (PRPP) concentration and erythrocyte PRPP synthetase (EC 2.7.6.1), hypoxanthine-guanine phosphoribosyltransferase (HGPRT; EC 2.4.2.8), adenine phosribosyltransferase (APRT; EC 2.4.2.7), adenosine deaminase (ADA; EC 3.5.4.4.), purine-nucleoside phosphorylase (PNP; EC 2.4.2.1)), transketolase (EC 2.2.1.1), and transaldolase (EC 2.2.1.2) activities were measured in the erythrocytes of 43 young hyperuricemic males and 7–13 normal male controls. In the hyperuricemic group a slightly elevated PRPP synthetase activity was observed, but there was a small decrease of the PRPP concentration. The transketolase activity was also slightly lower in the hyperuricemics. One hyperuricemic male was detected to have a partial APRT deficiency, and three were found to have elevated ADA activities. In all these cases an increased familial incidence of the abnormal enzyme activity was found. No HGPRT deficiency or PRPP synthetase hyperactivity was detected. |
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