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Energetics of photosynthesis in Zea mays. II. Studies of the flash-induced electrochromic shift and fluorescence induction in mesophyll chloroplasts |
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| Authors: | David Crowther Richard C Leegood David A Walker Geoffrey Hind |
| Institution: | 1. Biology Department, Brookhaven National Laboratory, Upton, NY 11973 U.S.A.;2. A.R.C. Research Group on Photosynthesis, Botany Department, The University, Sheffield S10 2TN U.K. |
| Abstract: | Patterns of electron transfer in isolated mesophyll chloroplasts of maize (Zea mays L.) were studied in the presence of the physiological substrates, oxaloacetate, 3-phosphoglycerate and pyruvate. Flash-induced absorbance changes due to the electrochromic pigment band-shift (P-518) were used to estimate relative electron flow rates through the cyclic and non-cyclic pathways of electron transport. Further information on the redox state of electron carriers and the activity of coupled electron flow was obtained from measurements of fluorescence induction and of actinic-light-induced fluorescence changes. The results demonstrate the importance of correct redox poising for optimal rates of photosynthesis and are discussed in relation to the operation and regulation of photosynthesis in the C4 system. |
| Keywords: | Photosynthesis Electron transport Chlorophyll fluorescence Fluorescence induction (Zea mays mesophyll chloroplast) DCMU 3-(3 4-dichlorophenyl)-1 1-dimethylurea Mes Tricine intrinsic bed fluorescence maximal level of fluorescence (seen in the presence of DCMU) Chl chlorophyll PS photosystem |
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