Demonstration of the formation of 1,3-diphosphoglyceric acid as an intermediate in the high-energy phosphate metabolism during reinitiation of development in Artemia

Extensive labelling of the glycolytic intermediate 2,3-diphosphoglycerate by ³²PO^3?₄ during the early periods of development in Artemia is reported. At 30 min of activation this is the major labelled compound. The mobilization of inorganic phosphate through glycolysis leading to the formation of 1,3-diphosphoglycerate results in the formation of a high-energy phosphate donor. The label from this compound could be chased to high-energy phosphates (adenine derivatives). The location and subsequent high degree of labelling of 2,3-diphosphoglycerate in the yolk platelets further demonstrate the important role played by this organelle in the metabolic events accompanying the breakdown of dormancy in Artemia.