Asymmetric or symmetric? Cytosolic modulation of human erythrocyte hexose transfer |
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Authors: | A Carruthers DL Melchior |
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Institution: | Department of Biochemistry, UMASS Medical Center, 55 Lake Avenue North, Worcester, MA 01605 U.S.A. |
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Abstract: | (1) The Michaelis-Menten parameters for hexose transfer in erythroctes, erythrocyte ghosts and inside-out vesicles at 20°C were determined using the light scattering method of Sen and Widdas ((1962) J. Physiol. 160, 392–403). (2) The external for infinite-cis exit of d-glucose in cells and ghosts is . (3) Dilution of cellular solute (up to × 90 dilution) by lysing and resealing cells in varying volumes of lysate is without effect on the for net d-glucose exit. The for net exit, however, falls from in intact cells to in ghosts. This effect is reversible. (4) Infinite-cis net d-glucose uptake measurements in cells and ghosts reveal the presence of a low , high affinity internal site of . The for net glucose entry increases from in intact cells to in ghosts. (5) The external for infinite-cisd-glucose exit in inside-out vesicles is . The kinetics of zero-transd-glucose exit from inside-out vesicles are changed markedly when cellular solute (obtained by lysis of intact cells) is applied to either surface of inside-out vesicles. When solute is present externally, the and for zero-trans exit are decreased by up to 10-fold. When solute is present at the interior of inside-out vesicles, for zero-trans exit is reduced; for exit is unaffected. In the nominal absence of cell solute, transfer is symmetric in inside-out vesicles. The orientation of transporter in the bilayer is unaffected by the vesiculation procedure. (6) External application of cellular solute to ghosts reduces for d-glucose exit but is without effect on the external for infinite-cis exit. (7) The inhibitory potency of cell lysate on hexose transfer is lost following dialysis indicating that the factors responsible for transfer modulation are low molecular weight species. (8) We consider the hexose transfer in human erythrocytes is intrinsically symmetric and that asymmetry of transfer is conferred by interaction of the system with low molecular weight cytosolic factors. |
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Keywords: | Glucose transport Hexose transfer Asymmetric transfer Inside-out vesicle (Human erythrocyte) |
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