Effects of nitrofen on chloroplast coupling factor-dependent reactions |
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Authors: | Bernhard Huchzermeyer Andreas Loehr |
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Institution: | Botanisches Institut, Tieraerztliche Hochschule, Buenteweg 17d, D-3000 Hannover 71 F.R.G. |
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Abstract: | Nucleotides induce a conformational change in the proteins of the CF0-CF1 complex. They give rise to reduced proton permeability of the thylakoid membranes. This reaction is paralleled by an enhanced yield of the steady-state proton uptake and a reduced nonphosphorylating electron-transport rate. Nitrofen acts as an energy-transfer inhibitor. It inhibits the rate of nucleotide exchange on CF1 both at ‘loose’ and ‘tight’ binding sites. During illumination the percentage of nucleotide-free CF0-CF1 complex seems to be enhanced in the presence of nitrofen. This results in a prevention of the described ADP effects on proton uptake and electron transport. These similar effects of nitrofen on loose and tight nucleotide-binding sites correspond with the idea that both types are different states of identical sites. |
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Keywords: | Photophosphorylation Herbicide Coupling factor Nitrofen (Spinach thylakoid) DCMU 3-(3 4-dichlorophenyl)-1 1-dimethylurea DCIP 2 6-dichlorophenolindophenol Tricine Chl chlorophyll |
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