Purification of microvillus membrane vesicles from pig small intestine by adsorption chromatography on sepharose |
| |
| Authors: | Jens Carlsen Kirsten Christiansen Birgit Bro |
| |
| Affiliation: | 1. Deparment of Biochemistry C, University of Copenhagen, Panum Institute, Blegdamsvej 3C, DK-2200 Copenhagen N Denmark;2. Deparment of Biochemistry A, University of Copenhagen, Panum Institute, Blegdamsvej 3C, DK-2200 Copenhagen N Denmark |
| |
| Abstract: | A simple, rapid method for the preparation of pure microvillus membrane vesicles from pig small intestine is described. The method is based on the ability of agarose beads to adsorb selectively the impurities, mainly basolateral membrane fragments, from a microvillus vesicle preparation isolated by hypotonic lysis, Mg2+ aggregation of contaminants and differential centrifugation. |
| |
| Keywords: | Vesicle purification Microvillus membrane Adsorption chromatography Sepharose (Pig intestine) |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
