Effect of poly(ethylene glycol) on the polarity of aqueous solutions and on the structure of vesicle membranes |
| |
Authors: | Andreas Herrmann Lothar Pratsch Klaus Arnold Günter Lassmann |
| |
Affiliation: | 1. Sektion Biologie der Humboldt-Universität, Bereich Biophysik, Invalidenstr. 42, 1040 Berlin G.D.R.;2. Sektion Physik der Karl-Marx-Universität, Bereich Molekülphysik, Linnestr. 5, 7010 Leipzig, Berlin G.D.R.;3. Zentralinstitut für Molekularbiologie der Akademie der Wissenschaften der DDR, Bereich Molekularbiophysik, Lindenberger Weg 70, 1115 Berlin G.D.R. |
| |
Abstract: | The partitioning of TEMPO into phosphatidylcholine vesicle membranes is reduced upon addition of poly(ethylene glycol). This is caused by reduced polarity of the aqueous phase as well as decreased membrane fluidity in the presence of poly(ethylene glycol). The isotropic hyperfine splitting of TEMPO in aqueous poly(ethylene glycol) solutions was used as a measure of solvent polarity. The alterations of the membrane fluidity were detected by means of two different fatty acid spin labels. The influences of physicochemical properties of an aqueous poly(ethylene glycol) phase on the membrane structure of cells and vesicles are discussed in the light of membrane fusion. |
| |
Keywords: | Membrane fusion Poly(ethylene glycol) Membrane fluidity Polarity ESR TEMPO 1-oxyl-2,2,6,6-tetramethylpiperidine DPPC I(10,3) I(1,14) 2-(14-carboxytetradecyl)-2-ethyl-4,4-dimethyl-3-oxazolidinyloxyl ANS 8-anilinonaphthalene 1-sulphonate |
本文献已被 ScienceDirect 等数据库收录! |
|