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Picosecond fluorescence kinetics in spinach chloroplasts at room temperature. Effects of phosphorylation |
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| Authors: | Philip Haworth Kerry K Karukstis Kenneth Sauer |
| Institution: | Department of Chemistry and Laboratory of Chemical Biodynamics, Lawrence Berkeley Laboratory, University of California, Berkeley, CA 94720 U.S.A. |
| Abstract: | We have used single-photon timing with picosecond resolution to investigate the effect of phosphorylation on the fluorescence decay from broken spinach chloroplasts. Phosphorylation of spinach thylakoids causes a quenching of the slow decay phase (equivalent to a quenching of variable fluorescence) and an increase in the yield of the middle phase decay component. In addition, phosphorylation alters the intensity dependence of fluorescence in a manner which indicates a decreased antenna size of Photosystem II. The observed changes are indicative of a State 1-State 2 transition and show a clear reversal when the membranes are dephosphorylated. |
| Keywords: | Photosynthesis Fluorescence decay Protein phosphorylation State 1-State 2 transition (Spinach chloroplast) PS photosystem Chl chlorophyll Tricine DCMU 3-(3 4-dichlorophenyl)-1 1-dimethylurea LHC light-harvesting chlorophyllprotein complex |
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