Effects of terbium on the hemocyanin pore formation rate in phosphatidylcholine planar bilayers |
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Authors: | Gianfranco Menestrina |
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Institution: | Dipartimento di Fisica, Universita'' Degli Studi di Trento, I-38050 Povo TN Italy |
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Abstract: | Megathura crenulata hemocyanin forms ionic channels in planar lipid bilayer membranes. It was found that hemocyanin is more potent as a channel former if TbCl3 is added to the bathing solution. Furthermore membranes separating symmetrical TbCl3 solutions show a pore formation rate which depends exponentially on the applied voltage, positive potentials favouring the insertion of new channels. The slope of this voltage dependence, which gives a measure of the effective charge displaced during the incorporation of one channel, increases and saturates with TbCl3 concentration. The dose response curve indicates that binding of Tb3+ to the phosphatidylcholine bilayer is involved in creating the effective charge. |
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Keywords: | Hemocyanin Model membrane Ion channel Pore formation rate Bistris 2-(bis(2-hydroxyethyl)amino)-2-(hydroxymethyl)-1 3-propanediol |
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