Solubilization of rat kidney benzodiazepine binding sites |
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Authors: | Claudia Martini Gino Giannaccini Antonio Lucacchini |
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Institution: | Instituto Policattedra di Discipline Biologiche, Università di Pisa, Via Bonanno 6, 56100 Pisa Italy |
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Abstract: | The high-affinity binding site for 3H]Ro 5–4864 has been solubilized from rat kidney using 1% Triton X-100. After lowering the concentration of detergent and using a poly(ethylene glycol) γ-globulin assay, it has been possible to demonstrate solubilization of about 90% of the binding sites. A single soluble class of binding sites with a of 1.8 nM is found. The order of potency of benzodiazepines is identical for the solubilized receptor and the membrane-bound form. Gel filtration revealed a major peak of binding activity with apparent molecular weight of 215000 and a Stokes' radius of 5.03 nm. |
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Keywords: | Benzodiazepine receptor Solubilization (Rat kidney) |
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