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Determination of the electric potential at the external and internal bilayer-aqueous interfaces of the human erythrocyte membrane using spin probes
Authors:Gregory S.B. Lin  Robert I. Macey  Rolf J. Mehlhorn
Affiliation:1. Department of Physiology-Anatomy, University of California, Berkeley, CA 94720 U.S.A.;2. Department of Biophysics and Medical Physics, University of California, Berkeley, CA 94720 U.S.A.;3. Lawrence Berkely Laboratory, University of California, Berkeley, CA 94720 U.S.A.
Abstract:Partitioning of oppositely charged amphipathic spin probes indicates that the electric potential at the external bilayer-aqueous interface of the human erythrocyte is insignificant, and that protruding sialic acids do not contribute to this potential. This potential at the surface is distinguished from the electrokinetic potential due to all charged groups within the hydrodynamic surface of shear. By contrast, using inside-out erythrocyte membrane vesicles, a substantial potential is observed at the cytoplasmic membrane surface. This can be attributed to the asymmetric distribution of acidic phospholipids on the two sides of the erythrocyte membrane bilayer.
Keywords:Surface potential  Spin probe  Erythrocyte membrane  Cell surface charge  Sialic acid  Inside-out vesicle  TEMPO  2,2,6,6-tetramethylpiperidine-1-oxyl  phospho-TEMPO  2,2,6,6-tetramethyl-4-phosphopiperidine-1-oxyl  TPS  2,4,6-triisopropylbenzenesulfonyl chloride
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