The effect of harmaline on unidirectional potassium fluxes and ouabain binding in renal cell cultures |
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Authors: | John H Becker John S Willis |
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Institution: | University of Illinois, Department of Physiology and Biophysics, 524 Burrill Hall, Urbana, IL 61801 U.S.A. |
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Abstract: | Harmaline inhibits K+ influx into primary cell cultures of ground squirrel kidneys to a greater extent than either ouabain or furosemide. A concentration of 200 μM harmaline was required to inhibit half of the total K+ influx; this effect was also seen at low temperature (5°C), and in another species (hamster). Although kinetic analysis of K+ influx indicates that harmaline does not compete with extracellular K+, harmaline did reduce the binding of 3H]ouabain to the cells. K+ efflux was also reduced. Therefore, harmaline may inhibit the furosemide-sensitive Na+/K+ cotransport system as well as the ouabain-sensitive Na+/K+ pump. |
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Keywords: | Harmaline Ouabain binding (Kidney cell culture) |
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