Effects of electric field on the photocycle of bacteriorhodopsin |
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Authors: | Sergei K Chamorovsky Eugenij P Lukashev Alexander A Kononenko Andrew B Rubin |
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Institution: | Department of Biology, Lomonosov State University, Moscow 117234 U.S.S.R |
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Abstract: | The photoconversion of bacteriorhodopsin and the effects of an applied electric field (5 · 107 V · m?1) were studied in dry films of purple membranes from Halobacterium halobium. The electric field was found to cause at least two different effects: (1) it blocks in part the formation of the batho-bacteriorhodopsin (K), most probably due to electrically-induced dark transition of some bacteriorhodopsin molecules into the photochemically inactive form; (2) it decreases the rate of the intermediate M decay, the rise time of the M formation being unaffected by electric field. The observed phenomena may suggest a feedback control mechanism for the regulation of the bacteriorhodopsin photocycle in purple membranes. |
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Keywords: | Bacteriorhodopsin Electric field effect Photocycle Purple membrane (H halobium) |
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