The use of confocal microscopy in the investigation of cell structure and function in the heart,vascular endothelium and smooth muscle cells |
| |
| Authors: | Bkaily Ghassan Pothier Pierre D'Orléans-Juste Pedro Simaan May Jacques Danielle Jaalouk Doris Belzile François Hassan Ghada Boutin Chantal Haddad Georges Neugebauer Witold |
| |
| Institution: | (1) MRCC Group on immuno-cardiovascular interactions, Department of Anatomy and Cell Biology, Faculty of Medicine, University of Sherbrooke, Sherbrooke, Quebec, J1H 5N4, Canada;(2) MRCC Group on immuno-cardiovascular interactions, Department of Pharmacology, Faculty of Medicine, University of Sherbrooke, Sherbrooke, Quebec, J1H 5N4, Canada |
| |
| Abstract: | In recent years, fluorescence microscopy imaging has become an important tool for studying cell structure and function. This non invasive technique permits characterization, localisation and qualitative quantification of free ions, messengers, pH, voltage and a pleiad of other molecules constituting living cells. In this paper, we present results using various commercially available fluorescent probes as well as some developed in our laboratory and discuss the advantages and limitations of these probes in confocal microscopy studies of the cardiovascular system. |
| |
| Keywords: | heart vascular endothelium vascular smooth muscle confocal microscopy pH calcium sodium voltage probe heart endothelin-1 Angiotensin II PAF nucleus mitochondria SR cardiomyopathy cells interaction R-type Ca2+ channel excitation-contraction coupling dystrophic mouse |
| 本文献已被 SpringerLink 等数据库收录！ |
|
