Time-dependent Alteration of Cytoskeletal Proteins in Cerebral Cortex of Rat During 2,5-Hexanedione-induced Neuropathy |
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Authors: | Fuyong Song Cuili Zhang Sufang Yu Xiulan Zhao Lihua Yu Keqin Xie |
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Institution: | (1) Institute of Toxicology, Shandong University, 44 West Wenhua Road, Jinan, Shandong, 250012, People’s Republic of China |
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Abstract: | To investigate the mechanisms of the axonopathy induced by 2,5-hexanedione (2,5-HD), male Wistar rats were administered at
a dosage of 400 mg/kg/day 2,5-HD (five times per week). The rats produced a slightly, moderately, or severely abnormal neurological
changes, respectively, after 2, 4, or 8 weeks of treatment. The cerebrums were Triton-extracted and ultracentrifuged to yield
a pellet fraction and a corresponding supernatant fraction. The relative levels of six cytoskeletal proteins (NF-L, NF-M,
NF-H, α-tubulin, β-tubulin, and β-actin) in both fractions were determined by immunoblotting. The results showed that NFs
content in HD-treated rats demonstrated a progressive decline as the intoxication of HD continued. As for microtubule proteins,
the levels of α-tubulin and β-tubulin demonstrated some inconsistent changes. The content of α-tubulin kept unchangeable,
while the content of β-tubulin increased significantly at the late stage of HD exposure. Furthermore, the content of β-actin
in both fractions remained unaffected throughout the study. These findings suggest that HD intoxication resulted in a progressive
decline of NFs, which was highly correlated with the development of HD-induced neuropathy. |
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Keywords: | 2 5-Hexanedione Neurofilaments Toxic axonopathy |
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