Stable expression of a fungal laccase protein using transplastomic tobacco |
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Authors: | Seyed Javad Davarpanah Joon-Woo Ahn Suk Min Ko Seo Hee Jung Youn-Il Park Jang Ryol Liu Won Joong Jeong |
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Institution: | 1. Green Bio Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 111 Gwahangno, Yuseong-gu, Daejeon, 305-806, Korea 3. Department of Biological Science, Chungnam National University, Daejeon, 305-764, Korea 2. Subtropical Horticulture Research Institute, Jeju National University, 66 Jejudaehakno, Jeju, 690-756, Korea
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Abstract: | Laccase catalyzes the oxidation of various phenolic compounds that can be used in a wide range of industrial applications such as waste detoxification and the textile industry. In the present study, we generated transplastomic tobacco plants to develop a reliable commercial source of laccase production. The stability of the laccase protein in the transgenic plants was increased by using the enhancer sequence from green fluorescent protein, resulting in three independent lines with high levels of laccase accumulation (up to 2?% of total protein); significant laccase activity, however, was not detected. Interestingly, the transplastomic lines showed slightly retarded vegetative growth, with a light green leaf color in comparison with the control, which may be attributable to copper deficiency induced by ligand chelation by abundantly produced laccase. These results suggest that the tobacco chloroplast is an efficient system for the mass production of laccase protein, but further studies are needed to obtain active enzyme. |
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