Microbial maximal specific growth rate as a square-root function of biomass yield and two kinetic parameters |
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| Authors: | Wilson W Wong James C Liao |
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| Institution: | 1. Department of Genetics, Faculty of Biology, Complutense University of Madrid, José Antonio Nováis 12, 28040 Madrid, Spain;2. Department of Plant Production: Botany and Plant Protection, EUIT Agrícola, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain;3. Department of Science and Technology Applied to Agricultural Engineering, EUIT Agrícola, Technical University of Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain;1. LBE, Univ Montpellier, INRA, 11100 Narbonne, France;2. MISTEA, Univ Montpellier, INRIA, Montpellier Supagro, 34060 Montpellier, France;3. MARBEC, Univ Montpellier, CNRS, IFREMER, IRD, 34203 Sète, France;2. University of Connecticut Health Center, Department of Cell Biology and Center for Cell Analysis and Modeling, Farmington, Connecticut;3. University of Arizona, Departments of Physics and Molecular and Cellular Biology, Tucson, Arizona;1. Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;2. Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai 201403, China;3. Agricultural Environment and Farmland Conservation Experiment Station of the Ministry of Agriculture, Shanghai 201403, China |
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| Abstract: | Understanding how growth rates changes under different perturbations is fundamental to many aspect of microbial physiology. In this work, we experimentally showed that maximal specific growth rate is a square-root function of the biomass yield, the substrate turnover number, and the maximum synthesis rate of the substrate transporter under that condition. We used Escherichia coli cultures in lactose minimal medium as a model system by introducing genetic modifications, in vitro evolution, and ethanol stress to the cell. Deletion of crr affected all three parameters in different directions while deletion of ptsG decreased only the biomass yield. Ethanol stress negatively impacted all three parameters, while anaerobicity decreased biomass yield and transporter synthesis rate. In addition, laboratory evolution increased the growth rate in lactose mostly through enhancing the expression rate of the lac operon. Despite all these changes, the growth rate of the perturbed strain was successfully related to the three parameters by the square-root equation. Thus, this square-root relationship provides insight into how growth rate is altered by different physiological parameters. |
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