RATE OF STEROL FORMATION BY RAT BRAIN GLIA AND NEURONS IN VITRO AND IN VIVO |
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Authors: | J P Jones H J Nicholas R B Ramsey |
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Institution: | Institute of Medical Education and Research, 1605 South Fourteenth Street, St. Louis, MO 63104, and Departments of Biochemistry and Neurology, St. Louis University School of Medicine, St. Louis, MO 63104, U. S. A. |
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Abstract: | The ability of 11-day-old rat glial and neuronal cells to biosynthesize sterol was studied as a function of time in vivo and in vitro. The in vitro experiments utilized 2-14C]mevalonic acid as precursor. Glial-enriched cell preparations demonstrated a greater ability to incorporate 2-14C]mevalonic acid into isoprenoid material than did neuronal-enriched preparations. Approximately 4 h were required for maximal uptake of labelled mevalonate by the glial preparations. Further metabolism of the isoprenoid material, involving squalene turnover and sterol demethylation, was still evident even after 15 h of incubation. In vivo, sterol biosynthesis was studied by intraperitoneal injection of sodium 2-14C]acetate and U-14C]glucose, sacrifice of the animals at 2 or 24 h, subsequent isolation of glial- and neuronal-cell enriched fractions and analysis of labelled isoprenoid material. Glial-enriched fractions again contained the bulk of the labelled isoprenoid material. |
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