Role of carbohydrates in rat leukemia cell-liver macrophage cell contacts |
| |
Authors: | J. Schlepper-Sch fer,N. Holl,V. Kolb-Bachofen,E. Friedrich,H. Kolb |
| |
Affiliation: | J. Schlepper-Schäfer,N. Holl,V. Kolb-Bachofen,E. Friedrich,H. Kolb |
| |
Abstract: | The mechanism by which macrophages recognize tumor cells is still unknown. We have studied interactions between rat liver macrophages and rat L 5222 leukemia cells. These tumor cells, but not normal leukocytes or erythrocytes, adhere to freshly isolated macrophages in vitro. Binding of tumor cells by macrophages can be inhibited by N-acetyl-D-galactosamine, D-galactose and more potently by glycoproteins with terminal N-acetyl-D-galactosamine or D-galactose residues. Tumor cell adhesion is calcium-dependent. The relevant leukemia cell membrane structures which bear terminal beta-D-galactosyl or related residues have been determined as trypsin- and pronase-sensitive, and hence may presumably be glycoproteins. The tumor cell receptor on liver macrophages appears to be a lectin with the carbohydrate specificity N-acetyl-D-galactosamine greater than D-galactose greater than L-fucose. |
| |
Keywords: | |
|
|