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In organello replication and viral affinity of linear, extrachromosomal DNA of the ascomycete Ascobolus immersus
Authors:F Kempken  F Meinhardt and K Esser
Institution:(1) Lehrstuhl für Allgemeine Botanik, Ruhr-Universität Bochum, Postfach 1021 48, D-4630 Bochum, Federal Republic of Germany;(2) Present address: Institut für Mikrobiologie, Westfälische Wilhelms-Universität, Corrensstrasse 3, D-4400 Münster, Federal Republic of Germany
Abstract:Summary Linear, extrachromosomal DNA's of the filamentous fungus Ascobolus immersus are localized within the mitochondria. These linear plasmids have no homology to the high molecular weight mtDNA (hmw mtDNA). For analysis of plasmid replication an in organello DNA synthesis system was developed, in which radionucleotides were incorporated into intact mitochondria. Plasmid DNA is labelled preferentially in this system. From replication analysis of a specific plasmid there is evidence of a virus-like protein-primed replication. Sequence analysis of this plasmid reveals that a viral DNA polymerase is encoded. Thus, these genetic elements presumably are viral remnants rather than true plasmids.
Keywords:Linear  mtDNA plasmids  In organello  virus-like replication  Viral DNA polymerase
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