Lateral release of proteins from the TOM complex into the outer membrane of mitochondria |
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Authors: | Max Harner Walter Neupert Marcel Deponte |
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Affiliation: | 1.Institut für Physiologische Chemie, Ludwig-Maximilians Universität, München, Germany;2.Max Planck Institut für Biochemie, Martinsried, Germany;3.Department für Parasitologie, Ruprecht-Karls Universität Heidelberg, Heidelberg, Germany |
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Abstract: | The TOM complex of the outer membrane of mitochondria is the entry gate for the vast majority of precursor proteins that are imported into the mitochondria. It is made up by receptors and a protein conducting channel. Although precursor proteins of all subcompartments of mitochondria use the TOM complex, it is not known whether its channel can only mediate passage across the outer membrane or also lateral release into the outer membrane. To study this, we have generated fusion proteins of GFP and Tim23 which are inserted into the inner membrane and, at the same time, are spanning either the TOM complex or are integrated into the outer membrane. Our results demonstrate that the TOM complex, depending on sequence determinants in the precursors, can act both as a protein conducting pore and as an insertase mediating lateral release into the outer membrane. |
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Keywords: | green fluorescent protein lateral release mitochondrial protein import protein localization protein translocase |
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