Dermaseptin 01 as antimicrobial peptide with rich biotechnological potential: study of peptide interaction with membranes containing Leishmania amazonensis lipid‐rich extract and membrane models |
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Authors: | Luiz C Salay Thatyane M Nobre Marcelle C Colhone Maria E D Zaniquelli Pietro Ciancaglini Rodrigo G Stabeli José Roberto S A Leite Valtencir Zucolotto |
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Institution: | 1. Instituto de Física de S?o Carlos, IFSC, Universidade de S?o Paulo, USP, 13560‐970 S?o Carlos, S?o Paulo, Brazil;2. Depto. Química, Faculdade de Filosofia, Ciências e Letras de Ribeir?o Preto (FFCLRP), Universidade de S?o Paulo, USP, 14040‐901 Ribeir?o Preto, S?o Paulo, Brazil;3. Centro de Estudos de Biomoléculas Aplicadas a Medicina, Núcleo de Saúde (NUSAU), Universidade Federal de Rond?nia (UNIR), 76800‐000, Porto Velho, RO, Brazil;4. Funda??o Oswaldo Cruz—Funda??o Oswaldo Cruz Noroeste (Fiocruz Noroeste), 76812‐245 Porto Velho, RO, Brazil;5. Núcleo de Pesquisa em Biodiversidade e Biotecnologia, Biotec, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaiba, Brazil |
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Abstract: | This article addresses the interactions of the synthetic antimicrobial peptide dermaseptin 01 (GLWSTIKQKGKEAAIAAA‐ KAAGQAALGAL‐NH2, DS 01) with phospholipid (PL) monolayers comprising (i) a lipid‐rich extract of Leishmania amazonensis (LRE‐La), (ii) zwitterionic PL (dipalmitoylphosphatidylcholine, DPPC), and (iii) negatively charged PL (dipalmitoylphosphatidylglycerol, DPPG). The degree of interaction of DS 01 with the different biomembrane models was quantified from equilibrium and dynamic liquid‐air interface parameters. At low peptide concentrations, interactions between DS 01 and zwitterionic PL, as well as with the LRE‐La monolayers were very weak, whereas with negatively charged PLs the interactions were stronger. For peptide concentrations above 1 µg/ml, a considerable expansion of negatively charged monolayers occurred. In the case of DPPC, it was possible to return to the original lipid area in the condensed phase, suggesting that the peptide was expelled from the monolayer. However, in the case of DPPG, the average area per lipid molecule in the presence of DS 01 was higher than pure PLs even at high surface pressures, suggesting that at least part of DS 01 remained incorporated in the monolayer. For the LRE‐La monolayers, DS 01 also remained in the monolayer. This is the first report on the antiparasitic activity of AMPs using Langmuir monolayers of a natural lipid extract from L. amazonensis. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd. |
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Keywords: | dermaseptin 01 antimicrobial peptides Leishmania amazonensis model membranes phospholipid monolayers dilatational surface elasticity nanomedicine for neglected diseases |
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