| 紫外线诱变和恒化器培养筛选耐高温的高产乳酸菌 |
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| 引用本文： | 鲁明波,曾翔,张力,卢正东,余龙江.紫外线诱变和恒化器培养筛选耐高温的高产乳酸菌[J].微生物学通报,2010,37(4):0520-0523. |
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| 作者姓名： | 鲁明波 曾翔 张力 卢正东 余龙江 |
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| 作者单位： | 华中科技大学分子生物物理教育部重点实验室,湖北武汉430074;华中科技大学生命科学与技术学院资源生物学与生物技术研究所,湖北武汉430074 |
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| 基金项目： | 国家863计划项目(No. 2008AA10Z339) |
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| 摘 要： | 本文探讨了利用紫外线诱变结合恒化器富集筛选耐高温高产乳酸菌的方法。首先以一株鼠李糖乳杆菌为出发菌种,并用紫外线的方法进行诱变,然后通过恒化器培养的方法在55oC下进行了耐高温高产菌株的富集。最终获得了9株耐高温菌株,其在高温下的产酸能力较出发菌株有了较大提高,其中一株突变株HT1发酵48h后L-乳酸产量达到了62.9g/L,比出发菌株提高了18.1g/L。本方法比一般平板筛选方法的效率高,大大减轻了复筛的工作量。
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| 关 键 词： | 乳酸菌 紫外线诱变 耐高温 恒化器富集 |
Ultraviolet Mutagenesis Combined Chemostat Enrichment Method to Screen High-temperature Resistent and High-yield Lactic Acid Bacteria |
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| LU Ming-Bo,ZENG Xiang,ZHANG Li,LU Zheng-Dong and YU Long-Jiang.Ultraviolet Mutagenesis Combined Chemostat Enrichment Method to Screen High-temperature Resistent and High-yield Lactic Acid Bacteria[J].Microbiology,2010,37(4):0520-0523. |
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| Authors: | LU Ming-Bo ZENG Xiang ZHANG Li LU Zheng-Dong and YU Long-Jiang |
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| Institution: | 1. Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; 2. Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China;1. Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; 2. Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China;1. Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; 2. Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China;1. Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; 2. Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China;1. Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China; 2. Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China |
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| Abstract: | This paper discusses the use of ultraviolet mutagenesis combined chemostat enrichment method to screen high-temperature resistent and high-yield lactic acid bacteria. Firstly, treated a strain of Lactobacillus rhamnosus with ultraviolet rays as mutagen, and then, before plate screening, used the chemostat continuous culture method to enrich high-temperature resistent and high-yield strains at 55oC. At last we obtained nine high-temperature strains. When fermented at 55oC, the highest production of L-Lactic acid was 62.9 g/L in 48 hours, which was 18.1 g/L higher than the starting strain. It was found that this method was more efficient than direct plate screening method by a comparing experiment, greatly reducing the workload of re-screening. |
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| Keywords: | Lactic acid bacteria Ultraviolet mutagenesis High-temperature resisted Chemostat Enrichment |
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