Non-neuronal cells of rat hypothalamus in dissociated cell culture |
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Authors: | Dr. Richard T. S. Worley Brian T. Pickering |
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Affiliation: | (1) Department of Anatomy, University of Bristol, The Medical School, Bristol, UK;(2) Department of Anatomy, University of Bristol, The Medical School, BS8 1TD Bristol, UK |
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Abstract: | Summary The neurophysin that is biosynthesised in association with the neurohypophysial hormone vasopressin (vasopressin-neurophysin) affects the growth and DNA synthesis of rat hypothalamic non-neuronal cells in culture. Over a narrow range of concentrations vasopressin-neurophysin stimulated growth, as assessed by increase in cell numbers, about five-fold, in conditions where fetal calf serum concentration was limiting (0.2% fetal calf serum). Maximum stimulation occurred in the presence of 20 to 30 ng vasopressin-neurophysin per ml of medium. DNA synthesis was increased by a factor of three in the presence of 30 ng vasopressin-neurophysin per ml of medium. At least two populations of non-neuronal hypothalamic cells were present in the cultures, and these were both affected by vasopressin-neurophysin.This study allows the suggestion that neurophysin may be acting as a growth-regulating factor at its release site, playing a part in the interactions of neurones and glial cells in the hypothalamo-neurohypophysial system. |
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Keywords: | Vasopressin-neurophysin Non-neuronal cells Cell culture Hypothalamus Growth factors |
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