The redox potential of liver cytochrome P-450 |
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| Authors: | M R Waterman H S Mason |
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| Affiliation: | 1. Department of Pharmacy, University of Malakand, Chakdara, Dir (Lower), KPK, Pakistan;2. Department of Pharmacy, University of Swabi, KPK, Pakistan;3. Faculty of Science, Taibah University, Almadina Almonawara, Saudi Arabia;4. Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Birkat Al Mauz 616, Nizwa, Sultanate of Oman;1. Veterinary and Animal Science Research Center (CECAV), University of Trás-os-Montes and Alto Douro, Quinta de Prados 500-801, Vila Real, Portugal;2. National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections, National Institute of Health Dr. Ricardo Jorge (NIH), Avenida Padre Cruz, 1600-560, Lisboa, Portugal;3. Department of Agriculture and Food, Area of Biochemistry and Molecular Biology, University of La Rioja (UR), 26006 Logroño, Spain;4. Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Quinta de Prados 500-801, Vila Real, Portugal;5. Functional Genomics and Proteomics Unit, University of Trás-os-Montes and Alto Douro, Quinta de Prados 500-801, Vila Real, Portugal;6. Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Quinta de Prados 500-801, Vila Real, Portugal;7. Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, Lisboa, 2829-516 Caparica, Portugal;8. Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland, UK;9. Research Institute of Biomedical and Health Sciences, University of Las Palmas de Gran Canaria, 35001 Canary Islands, Spain;10. Department of Food Hygiene and Technology, Veterinary Faculty, University of León, 24071 León, Spain;11. Institute of Food Science and Technology, University of León, 24071 León, Spain;12. DRRF, Direção Regional dos Recursos Florestais, Rua do Contador, 9500-050 Ponta Delgada, Azores, Portugal;13. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Universidade do Porto, 4099-002 Porto, Portugal;14. Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, 4099-002 Porto, Portugal;15. Centre for the Study of Animal Sciences (CECA/ICETA), Universidade do Porto, 4099-002 Porto, Portugal |
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| Abstract: | The redox potential of phenobarbital-induced rabbit liver cytochrome P-450 has been measured, within microsomes, using dyestuff reduction under anaerobic conditions, and optical and ESR spectrophotometric techniques for determining the ratios of oxidants and reductants in the system. An apparent midpoint potential of about −0.41 volt at pH 7.0 was found. The uncertainty concerning the exact E′o resulted from inability to achieve titration potentials by the dye-stuff technique, below −0.44 volt. |
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