生物肽SP对NK细胞活化性受体NCRs表达的影响

研究SP对NK92-MI细胞杀伤活性以及活化性受体NCRs(NKp46、NKp44和NKp30分子)的表达的影响,揭示SP对NK细胞杀伤功能的调节作用及其内在作用机制.MTT法测定NK92-MI细胞对K562细胞的杀伤活性；Real-Time PCR检测NCRs的mRNA表达；流式细胞术检测NCRs的膜表达.在10-14～10-8 mol/L浓度范围的SP作用24h,对NK92-MI细胞的杀伤活性有明显增强作用；10-14～10-8 mol/L的SP,均可增加NK92-MI细胞活化性受体NKp44、NKp46及NKp30的mRNA表达；该浓度范围的SP均可增加NKp46的膜表达水平,仅较低浓度( 10-14moL/L)的SP对NKp44的膜表达水平有增加作用,各浓度的SP对NKp30的膜表达水平均无明显影响.SP可通过上调活化性受体NCRs的表达水平来调节NK细胞的活性.

Effects on the Expression of Activation Receptors NCRs in NK cells by Bio-Peptide SP

The effects on cytotoxicity and expression of cell activation receptors NCRs(NKp46、NKp44 and NKp30) in NK92-MI cells by substance P(SP) were studied to reveal the mechanism of regulating NK cell activity and its inherent effective mechanism by SP.MTT assay was used to detect NK92-MI cell cytotoxicity;Real-Time PCR was used for detecting mRNA expression of NCRs;Using Flow cytometry to analyze membrane expression of NCRs.The results showed that SP at concentration of 10-14~10-8 mol/L treated for 24 h significantly enhanced the cytotoxicity of NK92-MI cell;SP at concentrations of 10-14~10-8 mol/L could increase all and significantly the expression of cell activation receptors NKp44,NKp46,and NKp30 mRNA in NK92-MI cells.In cells treated with SP at the concentration range,the membrane expression of NKp46 could significantly increase.Only a fairly low SP concentration(10-14 mol/L) had increasing effect on membrane expression of NKp44.However,various SP concentrations had no significant effect on membrane expression of NKp30.Therefore,SP could regulate NK cell activities through up-regulating the expression of activated receptor NCRs.