High efficiency <Emphasis Type="Italic">in vitro</Emphasis> plant regeneration from epicotyl explants of Chinese peanut cultivars |
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Authors: | Lei Shan Guiying Tang Pingli Xu Zhanji Liu Yuping Bi |
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Institution: | (1) High-Tech Research Center, Shandong Academy of Agricultural Sciences, Jinan, 250100, China;(2) Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Jinan, 250100, China;(3) Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan, 250100, China |
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Abstract: | An efficient organogenesis and micropropagation system was developed for in vitro plant regeneration of multiple cultivars of peanut (Arachis hypogaea). The system was used to regenerate plants from nine cultivars: Luhua no. 9, Luhua no. 13, Luhua no. 14, Fenghua no. 1, Fenghua
no. 3, Fenghua no. 5, Huayu no. 23, Qinglan no. 2, and Baisha 1016. Epicotyl and embryo axis explants were cultured on Murashige
and Skoog (MS) basal medium supplemented with 33.29–44.39 μM 6-benzyladenine (BAP) and 2.15–4.30 μM α-naphthaleneacetic acid
(NAA). The highest rate of shoot formation was observed in epicotyl explants taken from 4-d-old seedlings (5.1 ± 1.4 shoots
per explant). Optimum shoot development was observed in explants cultured on MS medium containing 4.48 μM BAP and 2.89 or
5.78 μM gibberellin (GA3). Well-developed shoots (3–5 cm high) formed roots after 2 wk on MS medium containing 0–2.69 μM NAA. We observed that all
multiple shoots formed at the site of epicotyl incision and at the upper end of each section, indicating physiological polarity
of shoot formation. The maximum shoot induction rate for Luhua no. 9, Luhua no. 13, Luhua no. 14, Fenghua no. 1, Fenghua no.
3, Fenghua no. 5, Huayu no. 23, Qinglan no. 2, and Baisha 1016 was 60.0%, 83.3%, 80.7%, 91.5%, 86.0%, 59.7%, 75.0%, 67.3%,
and 72.7%, respectively. This regeneration system will play a vital role in achieving the genetic improvement of Chinese peanut. |
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