Molecular and morphological approaches in discrimination of endangered <Emphasis Type="Italic">Probarbus jullieni</Emphasis> of Malaysia and Thailand stocks |
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Authors: | S Bhassu Z Abd Rashid |
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Institution: | (1) Department of Natural Resources and Environmental Science, University of Nevada, Reno, 1000 Valley Road, Reno, NV 89512, USA;(2) Geography Department, University of British Columbia, 1984 West Mall, Vancouver, BC, Canada;(3) Mekong Fish Conservation Project, Cambodia Department of Fisheries, 186 Norodom Blvd., P.O. Box 582, Phnom Penh, Cambodia |
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Abstract: | The population structure of Probarbus jullieni from Malaysia and Thailand stocks was based on seven microsatellite primers and truss network measurements. Truss morphometric
measurements were made on Temoleh, Probarbus jullieni to demonstrate the degree of speciation that can be induced by both biotic and abiotic conditions and contribute to the definition
of different stocks of Probarbus sp. At the momment no relevant information on stock definition has been produced recently concerning Probarbus spp., which is now in IUCN threatened red list. We also summarize the possible discriminant morphological characteristics
that shows differentiation between Malaysia and Thailand stocks. We also compare the levels of morphology and genetic differences
for Malaysian stocks throughout one year of sampling to determine whether sampling season and possible sexual dimorphism can
be detected in this fishes. A total of 25 different alleles were found across the two populations by the seven microsatellites,
of which 21 and 19 alleles were detected in Pahang, Malaysia and Thailand, respectively At the population level, the mean
number of alleles of Pahang (3.4991) per locus was higher than that (3.1665) of Thailand. From both molecular and morphometric
measurements showed that there were two distinct populations. However the differences between these two populations showed
that they belong to the same species with least degree of separation. |
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