Effect of Different Saccharides on Viability of Isolated Microspores and Androgenic Induction in Zea Mays |
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| Authors: | B Obert A Pre?ová B Büter JE Schmid |
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| Institution: | (1) Institute of Plant Genetic and Biotechnology, Slovak Academy of Sciences, Akademická 2, P. O. Box 39/A, SK 95007 Nitra, Slovak Republic;(2) Institute of Plant Science, Swiss Federal Institute of Technology (ETH) Zürich, Eschikon 33, CH 8315 Lindau, Switzerland |
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| Abstract: | Sucrose, glucose, fructose, and melibiose in different concentrations and combinations in the induction media influenced the viability of the isolated maize microspores and the formation of multinuclear structures. The induction of multinuclear structures on media containing combination of sucrose, fructose and glucose was lower than on media only with sucrose. In media containing melibiose alone or in combination with sucrose, no induction of multinuclear structures was found, however, microspore viability was improved. |
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| Keywords: | gametic embryogenesis haploid maize |
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