Type 1 and type 2 responses to Leishmania major |
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Authors: | Rogers Kathleen A DeKrey Gregory K Mbow M Lamine Gillespie R Dean Brodskyn Claudia I Titus Richard G |
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Institution: | School of Pharmaceutical Sciences, University of Nottingham, University Park, NG7 2RD, UK. |
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Abstract: | Pseudomonas aeruginosa and Burkholderia cepacia cause destructive lung disease in cystic fibrosis (CF) patients. Both pathogens employ 'quorum sensing', i.e. cell-to-cell communication, via diffusible N-acyl-L-homoserine lactone (AHL) signal molecules, to regulate the production of a number of virulence determinants in vitro. However, to date, evidence that quorum sensing systems are functional and play a role in vivo is lacking. This study presents the first direct evidence for the presence of AHLs in CF sputum. A total of 42 samples from 25 CF patients were analysed using lux-based Escherichia coli AHL biosensors. AHLs were detected in sputum from patients colonised by P. aeruginosa or B. cepacia but not Staphylococcus aureus. Furthermore, using liquid chromatography-mass spectrometry and thin layer chromatography, we confirmed the presence of N-hexanoylhomoserine lactone and N-(3-oxododecanoyl)homoserine lactone respectively in sputum samples from patients colonised by P. aeruginosa. |
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Keywords: | Quorum sensing Cystic fibrosis N-Acylhomoserine lactone Pseudomonas aeruginosa Burkholderia cepacia |
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