Activity and mRNA abundance of Delta-5 and Delta-6 fatty acid desaturases in two human cell lines |
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Authors: | de Antueno R J Allen S J Ponton A Winther M D |
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Institution: | QuantaNova Canada Ltd., P.O. Box 818, B4N 4H8, Kentville, N.S., Canada. r.deantueno@mailexcite.com |
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Abstract: | We analyzed fatty acid biosynthesis in Chang and ZR-75-1 cells. Both cell lines could desaturate and further elongate substrates for Delta-5 desaturase. ZR-75-1 but not Chang cells showed Delta-6 desaturation of 18:2n-6, 18:3n-3, 24:4n-6 and 24:5n-3. In both cell lines, the mRNA abundance can be related to Delta-5 or Delta-6 fatty acid desaturase activities. These results suggest that desaturase genes could have, at least in part, independent control mechanisms and that Delta-6 desaturase impairment is not specific to any particular step of the fatty acid metabolic pathways, which may diminish the rationale for the existence of at least two distinct enzymes. |
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