Influence of nitrogen source on growth and nitrogenase activity inAzotobacter vinelandii |
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Authors: | D Gadkari H Stolp |
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Institution: | (1) Institut für Allgemeine Botanik, Lehrstuhl für Mikrobiologie der Universität, Hamburg |
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Abstract: | Growth and nitrogenase activity were studied in cultures ofAzotobacter vinelandii growing with dinitrogen, ammonium sulfate, aspartic acid or yeast extract. Nitrogenase activity was measured by means of the C2H2 reduction test.In the presence of ammonium sulfate nitrogenase is completely repressed. After exhaustion of ammonia its activity is restored following a diauxic lag period of 30 min. With aspartic acid nitrogenase activity is partially repressed, and growth yield is higher than in the culture growing with N2 only. This is due to simultaneous use of dinitrogen and aspartate. Fluctuations of nitrogenase activity occurring during exponential growth and the mechanism of their regulation are discussed.Abbreviations NA
nitrogenase activity
- BNF
Burk's nitrogen free medium |
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Keywords: | Azotobacter vinelandii Nitrogenase Activity Acetylene Reduction Test Growth ofAzotobacter Regulation of Nitrogenase |
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