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Synthesis of simian virus 40 DNA in isolated nuclei

Authors:	P K Qasba

Institution:	Laboratory of Biochemistry, National Cancer Institute, National Institute of Health, Bethesda, Maryland 20014, USA

Abstract:	The presence or absence of calcium ions during the isolation of nuclei from SV40-infected African green monkey kidney cells significantly affects the size of SV40 DNA synthesized $in vitro$ . When Ca⁺⁺ is present during the nuclear isolation procedure, the 3–7S fragments of SV40 DNA synthesized $in vitro$ mature into long chains; in the absence of Ca⁺⁺ they do not.

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