25-hydroxyergocalciferol: a biologically active metabolite of vitamin D2 |
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| Authors: | T Suda H F DeLuca H Schnoes J W Blunt |
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| Affiliation: | 2. Department of Biochemistry and Molecular Biology, Federal University of Parana, Campus Centro Politecnico, CP 19046, 81531-980 Curitiba, PR, Brazil;1. Area of Nutrition and Food Sciences, Department of Molecular Biology and Biochemistry Engineering, Pablo de Olavide University, Carretera de Utrera Km 1, E-41013 Seville, Spain;2. Spanish Biomedical Research Centre in Diabetes and Associated Metabolic Disorders (CIBERDEM), Spain;3. Associated Researcher at Autónoma de Chile University, Av. Pedro de Valdivia 641, Santiago de Chile, Chile |
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| Abstract: | A polar, biologically active metabolite of vitamin D2 (ergocalciferol) has been isolated in pure form from the plasma of pigs fed 500,000 I.U. of vitamin D2 per day for 26 days. By means of its behavior during gas-liquid chromatography, ultraviolet spectra, mass spectrometry and nuclear magnetic spectrometry, its structure has been positively identified as 25-hydroxyergocalciferol. |
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