Activation of P2Y2 purinoceptor inhibits the activity of the Na+/K+-ATPase in HeLa cells |
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Authors: | Muscella Antonella Elia Maria Giovanna Greco Simona Storelli Carlo Marsigliante Santo |
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Affiliation: | Department of Biological Sciences and Environmental Technology (DisTeBA), Laboratory of Cell Physiology, University of Lecce, Ecotekne, Via Provinciale per Monteroni, 73100 Lecce, Italy. |
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Abstract: | The role of ATP on regulation of the Na+/K+-ATPase activity in the human cancerous HeLa cells was investigated. HeLa cells stimulated with increasing ATP concentrations showed a dose-dependent inhibition of the Na+/K+-ATPase activity. These effects were also obtained by UTP.ATP and UTP provoked a rise in intracellular calcium concentration ([Ca2+]i) persisting for at least 4 min. The inhibitor of phospholipase C, U73122, blocked the elevation of [Ca2+]i provoked by ATP/UTP. The expression of mRNA for P2Y2 and P2Y6 receptors was demonstrated by RT-PCR.ATP/UTP activated PKC-α, -βI and -ε isoforms, but not PKC-δ and -ζ. The inhibition of the Na+/K+-ATPase activity by ATP/UTP was blocked by Gö6976, a specific inhibitor of the calcium-dependent PKCs.In conclusion, our results suggest that ATP/UTP modulate Na+/K+-ATPase activity in HeLa cells through the P2Y2 purinoceptor via calcium mobilisation and activation of calcium-dependent PKCs. |
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