Proteomic analysis of soybean nodule cytosol |
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Authors: | Oehrle Nathan W Sarma Annamraju D Waters James K Emerich David W |
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Institution: | a United States Department of Agriculture, Curtis Hall, University of Missouri, Columbia, MO 65211, United States b Department of Biochemistry, 117 Schweitzer Hall, University of Missouri, Columbia, MO 65211, United States c Agricultural Experiment Station Chemical Laboratories, 4 Agriculture Building, University of Missouri, Columbia, MO 65211, United States |
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Abstract: | An isolation procedure for soybean (Glycine max L. cv Williams 82) nodule cytosol proteins was developed which greatly improved protein resolution by two-dimensional polyacrylamide gel electrophoresis. The most abundant proteins were selected and analyzed by mass spectrometry. The identified proteins were categorized by function (% of total proteins analyzed): carbon metabolism (28%), nitrogen metabolism (12%), reactive oxygen metabolism (12%) and vesicular trafficking (11%). The first three categories were expected based on the known physiological functions of the symbiotic nitrogen fixation process. The number of proteins involved in vesicular trafficking suggests a very active exchange of macromolecules and membrane components. Among the 69 identified proteins were the enzymes of the three carbon portion of glycolysis, which were further characterized to support their roles in the sucrose synthase pathway to provide malate for the bacteroids. Proteomic analysis provides a functional tool by which to understand and further investigate nodule function. |
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Keywords: | Glycine max Leguminosae Proteomics Soybean Nodules Bradyrhizobium japonicum Glycolysis 2D Electrophoresis Mass spectrometry |
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