| 双歧杆菌的完整肽聚糖对小鼠腹腔巨噬细胞膜脂流动性的影响 |
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| 引用本文： | 魏晓霞,李迎雪,王立生,荀安营,朱惠明,马晓东. 双歧杆菌的完整肽聚糖对小鼠腹腔巨噬细胞膜脂流动性的影响[J]. 中国微生态学杂志, 2008, 20(4): 335-336 |
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| 作者姓名： | 魏晓霞 李迎雪 王立生 荀安营 朱惠明 马晓东 |
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| 作者单位： | 1. 暨南大学第二临床医学院,深圳市人民医院,消化科,广东,深圳,518020
2. 南方医科大学,中心实验室,广东,广州,510515 |
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| 摘 要： | 目的探讨分叉双歧杆菌的完整肽聚糖（WPG）对巨噬细胞膜脂流动性的影响。方法首先分离培养昆明小鼠腹腔巨噬细胞,然后以WPG刺激巨噬细胞,再用细胞膜磷脂荧光探针标记细胞,最后采用激光共聚焦显微镜结合激光漂白后荧光恢复技术检测巨噬细胞的膜脂流动性。结果WPG刺激组反映小鼠腹腔巨噬细胞膜脂流动性的平均荧光恢复率明显高于对照组（P〈0.01）。结论分叉双歧杆菌的完整肽聚糖可提高巨噬细胞膜脂流动性。
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| 关 键 词： | 双歧杆菌 完整肽聚糖 巨噬细胞 膜脂流动性 |
Influence of whole peptidoglycan of Bifidobacteria on the membrane lipidic fluidity of murine peritoneal macrophages |
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| WEI Xiao-xia,LI Ying-xue,WANG Li-sheng,XUN An-ying,ZHU Hui-ming,MA Xiao-dong. Influence of whole peptidoglycan of Bifidobacteria on the membrane lipidic fluidity of murine peritoneal macrophages[J]. Chinese Journal of Microecology, 2008, 20(4): 335-336 |
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| Authors: | WEI Xiao-xia LI Ying-xue WANG Li-sheng XUN An-ying ZHU Hui-ming MA Xiao-dong |
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| Affiliation: | WEI Xiao-xia , LI Ying-xue , WANG Li-sheng , XUN An-ying, ZHU Hui-ming , MA Xiao-dong ( 1. Department of Gastroenterology, Shenzhen People＇ s Hospital, the Second Clinical Medical College, Jinan University, Shenzhen 518020, China ; 2. Central Laboratory, Southern Medical University, Guangzhou 510515, China) |
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| Abstract: | Objective To explore the influence of whole peptidoglycans （WPG） of Bifidobacteria bifidum on the membrane lipidic fluidity of murine peritoneal macrophages. Methods Mouse peritoneal macrophages were separated and cultivated. The macrophages were stimulated by WPG, and labelled by the fluorescence probe of the membrane lipid. The membrane lipidic fluidity of the macrophages was detected by using laser scanning confocal microscopy and fluorescence re- covery after photobleaching. Result The average fluorescence recovery ratio of the macrophages in WPG stimulating group was significantly higher than that in control group. Conclusion WPG of Bifidobacteria bifidum could increase the mem- brane lipidic fluidity of murine peritoneal macrophages. |
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| Keywords: | Bifidobacterim Whole peptidoglycan Macrophage Membrane lipidic fluidity |
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