Expression of 10 kD Sulfur-Rice Prolamin Gene of Rice in Potato

Using 10 kD sulfur-rich prolamin gene of rice (PLG) as target gene, the authers constructed the expression vectors pBinLG and pBinLGP, which contained CaMV 35S promoter/PLG/NOS terminator, and Patafin Class Ⅰ promoter/PLG/NOS terminator respectively. They were transformed into Agrobacterium tumefaciens strain LBA4404 (pAL4404) by direct transformation method with incubating the leaf and tuber explants of potato (Solanum tuberosum L. ) with LBA4404 (pAL4404) and selecting in the medium containing 100 mg/L kanamycin, regenerated resistant plants were obtained. The NPT Ⅱ enzyme activity analysis, polymerase chain reaction (PCR), Southern blotting, Northern dot blotting and Western blotting demonstrated that the target gene was integrated into the genome of potato ceils and well expressed in the plant.