| Abstract: | Antiserum against human VnR integrin, and ay, β3 integrin subunit cytoplasmic domain in Western blots were applied to determine if integrin-like proteins be present in the pollen tube membrane of Hemerocallis citrina Baroni. The results showed that anti-β3 integrin subunit serum could recognize 140 kD and 97 kD bands in SDS-PAGE gels under the reducing conditions, while antiserum against VnR and ay integrin could recognize 160 kD and 155 kD bands respectively under the reducing conditions, and also two small bands of higher molecular weight under the non-reducing conditions. Non-immune semm control could not cross react with any protein bands. The present study suggest that the integTin-like protein, the receptor of vitronecttn could exist in the form of av and β3 subunits in the pollen tube membrane, with its molecular mass quite similar to that of the integrin reported in animal. |
