| Abstract: | Primary somatic embryogenesis and shoot organogenesis in vitro could be directly induced from immature leaves of cassava ( Manihot esculenta Crantz) with higher concentration ( 10 to 80 mg/L) of NAA. Compared with 4 mg/L 2,4-D on the induction and regeneration system, NAA showed some advantageous characteristics, that is, NAA could direcfiy induce both primary somatic embryogenesis and shoot organogenesis, whereas 2,4-D could only induce somatic embryogenesis. NAA induced somatic embryogenesis much quicker, producing visible somatic embryos within 9 to 13 days and shoot (tips) within 10 to 14 days, than 2,4-D, which would induce visible somatic embryos after 15 days. Plant regeneration from the NAA-induced somatic embryos was as high as 48%, but was only 4.1% from that of 2,4-D. The test also showed that primary somatic embryogenesis or shoot organogenesis could be induced directly from immature leaves in 12 out of 16 cassava varieties. |
