Immobilisation of anchorage-independent animal cells using reticulated polyvinyl formal resin biomass support particles |
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Authors: | Hideki Yamaji Hideki Fukuda Yasuhiro Nojima Colin Webb |
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Institution: | (1) Engineering Research Laboratories, Kanegafuchi Chemical Industry Co., Ltd., 1-8 Miyamae, 676 Takasago, Hyogo, Japan;(2) Department of Chemical Engineering, UMIST, Sackville Street, P. O. Box 88, M60 1QD Manchester, United Kingdom |
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Abstract: | Summary Immobilisation of anchorage-independent animal cells using Biomas Support Particles (BSPs) was investigated. Mouse myeloma MPC-11 cells were physically entrapped in three-dimensional reticulated polyvinyl formal (PVF) resin PSPs (3×3×3 mm) with matrices of relatively small pores (30–100 m) by filtering medium containing cells or incubating in a shake flask for inoculation. Physically entrapped cells became immobilised in the BSPs by forming aggregates within the matrices of the reticulated PVF resin, and cell density in the BSPs reached at least 107 cells/cm3 BSP. Immobilised cells in the BSPs were successfully cultivated in static and/or shake-flask cultures with regular replacement of medium for a long period. |
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