Patterns of ligninolytic enzymes in Trametes versicolor. Distribution of extra- and intracellular enzyme activities during cultivation on glucose, wheat straw and beech wood |
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Authors: | D Schlosser R Grey W Fritsche |
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Institution: | (1) Institute of Microbiology, University of Jena, Philosophenweg 12, D-07745 Jena, Germany Fax: 03641-63 12 37 e-mail: Schlosser@merlin.biologie.uni-jena, DE |
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Abstract: | Trametes versicolor was shown to produce extracellular laccase during surface cultivation on glucose, wheat straw and beech wood. Growth on both
wheat straw and beech wood led to an increase as high as 3.5-fold in extracellular laccase activity, in comparison with growth
on glucose. The corresponding yields in fungal biomass reached only about 20% of the value obtained on glucose. Manganese
peroxidase activity␣appeared during growth on wheat straw and beech wood. Mycelia grown on glucose, wheat straw and beech
wood also showed intracellular laccase activities, monitored with 2,6-dimethoxyphenol, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic
acid), 4-hydroxy-3,5-dimethoxybenzaldehyde azine (syringaldazine) and 3,4-dihydroxyphenylalanine (l-DOPA). Assaying intracellular laccase with 2,6-dimethoxyphenol, syringaldazine and l-DOPA showed the maximum oxidation rates to be at pH values different from those producing maximum oxidation rates with extracellular
laccase. In each case most of the total laccase activity was recovered from the culture filtrates. Growth on wheat straw and
beech wood led to increased values for both extra- and intracellular laccase activities, based on fungal dry weight, in comparison
with growth on glucose.
Received: 18 July 1996 / Received revision: 19 November 1996 / Accepted: 23 November 1996 |
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