Developing genetic reagents to facilitate recovery, analysis, and maintenance of mouse mutations |
| |
Authors: | Eugene M Rinchik |
| |
Institution: | (1) Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831-8077, and Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee–Knoxville, Knoxville, Tennessee 37996, USA, US |
| |
Abstract: | Because the mouse has become the pre-eminent model system for functional genomics and analysis of complex-systems/pathways
in mammals, there has been an escalation of interest in the generation and analysis of mouse mutations to use as tools in
these analyses. I argue here for a parallel investment in continuing the development of appropriately marked chromosomal rearrangements
to use as genetic reagents in mutation recovery, analysis, and maintenance crosses. Specifically, visibly marked interstitial
chromosomal deletions can be valuable for regional mutagenesis screens for recessives based on hemizygosity, and they can
also be used to simplify genetic fine-mapping as a prelude to gene identification based on positional cloning/candidacy strategies.
Dominantly marked chromosomal inversions that also manifest some kind of recessive phenotype can be exploited in more extensive
regional mutagenesis screens based on homozygosity, and are invaluable for simplified, low-cost and error-reduced mutant-stock
maintenance. Also discussed are several issues concerning genetic background, particularly from the point of view of genetic-reagent
resource development.
Received: 16 December 1999 / Accepted: 16 December 1999 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|