Effects of FADS and ELOVL polymorphisms on indexes of desaturase and elongase activities: results from a pre-post fish oil supplementation |
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Authors: | Hubert Cormier Iwona Rudkowska Simone Lemieux Patrick Couture Pierre Julien Marie-Claude Vohl |
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Affiliation: | .Institute of Nutrition and Functional Foods (INAF), Laval University, 2440 Hochelaga Blvd, Québec, QC G1V 0A6 Canada ;.School of Nutrition, Laval University, Quebec City, G1K 7P4 Canada ;.Department of Kinesiology, Laval University, Quebec City, G1V 0A6 Canada ;.Endocrinology and Nephrology, CHU de Québec Research Center, Québec, G1V 4G2 Canada |
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Abstract: | Polymorphisms (SNPs) within the FADS gene cluster and the ELOVL gene family are believed to influence enzyme activities after an omega-3 (n-3) fatty acid (FA) supplementation. The objectives of the study are to test whether an n-3 supplementation is associated with indexes of desaturase and elongase activities in addition to verify whether SNPs in the FADS gene cluster and the ELOVL gene family modulate enzyme activities of desaturases and elongases. A total 208 subjects completed a 6-week supplementation period with 5 g/day of fish oil (1.9–2.2 g/day of EPA + 1.1 g/day of DHA). FA profiles of plasma phospholipids were obtained by gas chromatography (n = 210). Desaturase and elongase indexes were estimated using product-to-precursor ratios. Twenty-eight SNPs from FADS1, FADS2, FADS3, ELOVL2 and ELOVL5 were genotyped using TaqMan technology. Desaturase indexes were significantly different after the 6-week n-3 supplementation. The index of δ-5 desaturase activity increased by 25.7 ± 28.8 % (p < 0.0001), whereas the index of δ-6 desaturase activity decreased by 17.7 ± 18.2 % (p < 0.0001) post-supplementation. Index of elongase activity decreased by 39.5 ± 27.9 % (p < 0.0001). Some gene–diet interactions potentially modulating the enzyme activities of desaturases and elongases involved in the FA metabolism post-supplementation were found. SNPs within the FADS gene cluster and the ELOVL gene family may play an important role in the enzyme activity of desaturases and elongases, suggesting that an n-3 FAs supplementation may affect PUFA metabolism. |
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Keywords: | Omega-3 fatty acids, Diet and dietary lipids, Genomics, Fish oil, Omega-6 fatty acids, Genotype |
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