Determination of c-Abl tyrosine kinase and mTERT catalytic subunit of telomerase expression level during prenatal-postnatal mouse ovary-testis development |
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| Affiliation: | 1. Department of Obstetrics and Gynecology, Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Istanbul, Turkey;2. Department of Obstetrics and Gynecology, Reyap Hospital Corlu, Tekirdag, Turkey;3. Department of Biochemistry, Yildiz Technical University, Istanbul, Turkey;4. Department of Biochemistry, Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Istanbul, Turkey;5. Department of Obstetrics and Gynecology, Memorial Bahcelievler Hospital, Istanbul, Turkey;6. Department of Physiology, Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Istanbul, Turkey;1. Departments of Zoology, Faculty of Science, Damietta University, Egypt;2. Departments of Botany & Microbiology, Faculty of Science, Damietta University, Egypt;1. Department of Reproductive Center, First Affiliated Hospital ofGuangxi Medical University, Nanning, Guangxi, 530021, China;2. Department of Obstetrics and Gynecology, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, 545005, China;3. Reproductive Medicine Center, Liuzhou Maternal and Child Healthcare Hospital, Liuzhou, Guangxi, 545005, China;1. Shanghai JIAI Genetics & IVF Institute, Obstetrics & Gynecology Hospital of Fudan University, Shanghai 200011, China;2. Shanghai Key Laboratory for Female Reproductive Endocrine-Related Diseases, Institute of Reproductive and Development, Fudan University, Shanghai 200433, China;1. Department of Animal Reproduction and Veterinary Radiology, College of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, Brazil;2. Paranaense University, Department of Animal Reproduction, Umuarama, Paraná, Brazil;3. Santo Amaro University, Department of Animal Reproduction, São Paulo, São Paulo, Brazil;4. Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil;1. Constantine the Philosopher University, 949 74, Nitra, Slovakia;2. Research Institute of Animal Production, National Agricultural and Food Center, 951 41 Lužianky, Slovakia;3. King Saud University, College of Science, Zoology Department, 11451, Riyadh, Saudi Arabia |
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| Abstract: | Expression levels of genes involved in the development of germ cells vary throughout the process from bipotential gonadal period to adult gonadal formation. In mice, developments of female and male reproductive system are regulated by germ cell-specific factors and hormones, and determinative days in this regulation are very important. c-Abl is a non-receptor tyrosine kinase with cellular functions including cell proliferation, growth and development. mTERT is involved in maintaining telomerase activity and proliferation of surviving cells. We suggested that c-Abl and mTERT might be important for the healthy development of prenatal and postnatal mouse ovary and testis. We aim to demonstrate localization and expressions of c-Abl and mTERT in crucial days of ovary and testis development in prenatal and postnatal period in mouse by immunofluorescence staining and qRT-PCR, respectively. The importance of c-Abl and mTERT expressions during the healthy gonadal development is indicated in the prenatal and postnatal gonadal development. Also, protein expression levels were detected by Western Blot in only postnatal ovary and testis. Determining the functions of the c-Abl and mTERT throughout the process will be important in terms of understanding the infertility cases in the female and male with future studies. |
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| Keywords: | c-Abl mTERT ovary testis development |
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