Puncturevine (Tribulus terrestris L.) affects the proliferation,apoptosis, and ghrelin response of ovarian cells |
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| Institution: | 1. Constantine the Philosopher University, 949 74, Nitra, Slovakia;2. Research Institute of Animal Production, National Agricultural and Food Center, 951 41 Lužianky, Slovakia;3. King Saud University, College of Science, Zoology Department, 11451, Riyadh, Saudi Arabia;1. Department of Reproductive Center, First Affiliated Hospital ofGuangxi Medical University, Nanning, Guangxi, 530021, China;2. Department of Obstetrics and Gynecology, the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, 545005, China;3. Reproductive Medicine Center, Liuzhou Maternal and Child Healthcare Hospital, Liuzhou, Guangxi, 545005, China;1. Shanghai JIAI Genetics & IVF Institute, Obstetrics & Gynecology Hospital of Fudan University, Shanghai 200011, China;2. Shanghai Key Laboratory for Female Reproductive Endocrine-Related Diseases, Institute of Reproductive and Development, Fudan University, Shanghai 200433, China;1. Departments of Zoology, Faculty of Science, Damietta University, Egypt;2. Departments of Botany & Microbiology, Faculty of Science, Damietta University, Egypt;1. University of Veterinary Medicine, Department of Obstetrics and Reproduction, István str 2., H-1078 Budapest, Hungary;2. Department of Anatomy, University of Pecs Medical School, MTA-PTE PACAP Research Team, Szigeti str 12., H-7624 Pécs, Hungary;1. Department of Animal Reproduction and Veterinary Radiology, College of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, Brazil;2. Paranaense University, Department of Animal Reproduction, Umuarama, Paraná, Brazil;3. Santo Amaro University, Department of Animal Reproduction, São Paulo, São Paulo, Brazil;4. Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, Distrito Federal, Brazil;1. Human Cytogenetics, National Research Center, 12622, Cairo, Egypt;2. Department of Zoology, Faculty of Science, Minia University, 61519, El Minia, Egypt;3. Department of Clinical Genetics, National Research Center, 12622, Cairo, Egypt |
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| Abstract: | The objective of our study was to examine the direct effects of the medicinal plant Tribulus terrestris L. (puncturevine) on the basic functions of ovarian cells, including their proliferation, apoptosis, and response to the physiological hormonal stimulator ghrelin. In the first series of experiments, porcine ovarian granulosa cells were cultured with or without puncturevine extracts at concentrations of 0, 1, 10, or 100 μg/ml. In the second series of experiments, these cells were cultured with ghrelin at concentrations of 0, 1, 10, or 100 ng/ml, either alone or in combination with puncturevine (10 μg/ml). The expression levels of the proliferation marker PCNA and the apoptosis marker bax were analyzed via quantitative immunocytochemical methods. Puncturevine was found to stimulate the accumulation of both proliferation and apoptotic markers. Additionally, ghrelin alone could promote the proliferation and apoptosis of ovarian cells. The presence of puncturevine reversed ghrelin-stimulated apoptosis and instead induced apoptotic inhibition. However, puncturevine did not modify the proliferation-inducing effect of ghrelin. These observations demonstrated that (1) puncturevine directly promotes cell proliferation and apoptosis, turnover, of ovarian cells; (2) ghrelin is involved in the regulation of ovarian cell apoptosis and proliferation, consistent with existing evidence; (3) puncturevine antagonizes and even reverses the effects of the hormonal regulator, ghrelin, on ovarian cell apoptosis, but not proliferation; and (4) puncturevine affects not only the basic functions of ovarian cells but also their responses to upstream hormonal regulators. |
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| Keywords: | Ovary Proliferation Apoptosis Ghrelin |
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