Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves |
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Authors: | Junior Cesar Casagrande Luis Fernando Benitez Macorini Katia Avila Antunes Uilson Pereira dos Santos Jaqueline Ferreira Campos Nelson Miguel Dias-Júnior Andréia Sangalli Claudia Andrea Lima Cardoso Maria do Carmo Vieira Luiza Antas Rabelo Edgar Julian Paredes-Gamero Edson Lucas dos Santos Kely de Picoli Souza |
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Affiliation: | 1. Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil.; 2. State University of Mato Grosso do Sul, Dourados, Mato Grosso do Sul, Brazil.; 3. Federal University of Alagoas, Maceió, Alagoas, Brazil.; 4. Federal University of São Paulo, São Paulo, São Paulo, Brazil.; University of Lancaster, United Kingdom, |
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Abstract: | Background and PurposeLeaves of Jacaranda decurrens are used in traditional Brazilian medicine to treat metabolic diseases related to increased reactive oxygen species. The present study evaluated the antioxidant and cytotoxic potential of hydroethanolic extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata.Experimental ApproachPhenolic compounds, flavonoids and saponins were evaluated in an ethanol∶water (80∶20, v/v) extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata (E-Jds). The antioxidant activity of E-Jds was investigated by assessing the following: 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity; protection against 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis of erythrocytes; in vitro and in vivo malondialdehyde dosage; and the ability to activate antioxidant enzymes. K562 leukemia cells were used for the cytotoxic evaluation of E-Jds and for the assessment of the cell death profile through flow cytometry.Key ResultsPhenolic and flavonoid compounds were quantified as 14.38% and 2.15%, respectively, of E-Jds. These phenolic and flavonoid compounds proved to be able to scavenge DPPH free radicals with an IC50 of 9.3±3.3 µg/mL, to protect up to 50% of erythrocytes against AAPH-induced hemolysis and to reduce in vitro and in vivo malondialdehyde levels up to 84% and 22%, respectively. E-Jds also increased glutathione peroxidase enzyme activity, with a concomitant decrease in superoxide dismutase and catalase activity, and exhibited dose-dependent cytotoxic activity on K562 erythroleukemia cells with cell death occurring via both late apoptosis and necrosis.ConclusionsE-Jds exhibits in vitro and in vivo antioxidant potential, which may be the mechanism mediating the metabolic activities reported in folk medicine. Furthermore, the cytotoxic activity identified in this study contributes with the knowledge of antiproliferative activities that have been described in the literature for the genus Jacaranda. |
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