Mouse embryos exposed to oxygen concentrations that mimic changes in the oviduct and uterus show improvement in blastocyst rate,blastocyst size,and accelerated cell division |
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| Affiliation: | 1. Dep. Gyn. Endocrinology and Reproductive Medicine, University Hospital Giessen, Marburg, Germany;2. Institute of Medical Biometry and Epidemiology, Philipps University, Marburg, Germany;3. Clinic for Veterinary Obstetrics, Gynaecology, and Andrology, Chair for Molecular Reproductive Medicine, Justus-Liebig-University, Giessen, Germany;1. Departamento de Ciências Naturais, Universidade Federal de São João del Rei. Praça Dom Helvécio, 74 - Dom Bosco, São João del-Rei, MG, 36301-160, Brazil;2. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, Belo Horizonte, MG, 31270-901, Brazil;3. Departamento de Zootecnia, ESALQ/Universidade de São Paulo, Av. Pádua Dias 11, Piracicaba, SP, 13418-900, Brazil;4. Embrapa Recursos Geneticos e Biotecnologia, PqEB, Av. W5 Norte, Brasília, DF, 70770-917, Brazil;1. The Fertility Center from A to Z, Affiliated with Assuta Medical Center, Tel Aviv, Israel;2. The Fertility Clinic, Skive Regional Hospital, Denmark;3. Faculty of Health, Aarhus University, Denmark;4. The Sackler Faculty of Medicine, Tel Aviv University, P.O.B 39040, Tel Aviv, Israel;1. Department of Urology, Krankenhaus Hietzing mit Neurologischem Zentrum Rosenhügel, Vienna, Austria;2. Department of Laboratory Medicine, Krankenhaus Hietzing mit Neurologischem Zentrum Rosenhügel, Vienna, Austria;3. Karl Landsteiner Institute for Clinical Risk Management, Vienna, Austria;4. Department of Urology, Royal Bournemouth and Christchurch Hospitals NHS Foundation Trust, Bournemouth, United Kingdom;5. Department of Surgery, University of Cambridge, Cambridge, United Kingdom;1. Faculty of Medicine, Universiti Teknologi MARA Sungai Buloh Campus, Selangor, Malaysia;2. I-PPerFORM, Universiti Teknologi MARA, Malaysia;1. Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran;2. Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran;3. Neurobiology Research Group, Faculty of Life Sciences, The University of Manchester, Manchester, UK |
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| Abstract: | After in vivo fertilisation, the preimplantation embryo goes through cleavage during migration along the oviduct in mammals or the fallopian tube in a woman and ends up inside the uterus. This study investigates the effect of a protocol aimed at closely reproducing that natural oxygen concentration in the oviduct (7 % O2 from day 1 to day 3 and 2 % from day 3 to day 5), in contrast to the concentrations (5 % or 20 %) widely used in practice in ART using morphokinetic. Female mice (BI6/CBAca) were sacrificed, and zygotes were isolated 20 h after mating and randomly allocated to three parallel groups, which were grown under high atmospheric, low, or sequential oxygen concentrations. Zygotes were cultured in GTL medium (Vitrolife) and observed by the Primovision time-lapse system. Blastocyst rate at 120 h in the sequential group (91.3 %) was significantly increased over the high (76.3 %) and low (74.4 %) groups. Blastocyst size was also enlarged in the sequential group compared to the high and low groups. Moreover, cell division in the sequential group was significantly faster at almost every cleavage stage than it was in the other groups. Notably, the duration of the interims between stages also differed significantly between the groups. This study demonstrated that, in comparison to routinely used high or low oxygen conditions, oxygen concentrations mimicking changes in the oviduct and uterus significantly improve the blastocyst rate and size and accelerate cell division at several stages as well as the interims between cleavage events. |
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| Keywords: | Oxygen concentrations Blastocyst development Mouse embryo assay Morphokinetic Time-lapse imaging |
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