Chronic unpredictable stress-induced reproductive deficits were prevented by probiotics |
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| Affiliation: | 1. Key Laboratory of Family Planning and Reproductive Genetics, National Health and Family Planning Commission, Shijiazhuang, 050071, China;2. College of Life Science, Hebei Normal University, Shijiazhuang, 050024, China;1. Faculty of Medicine, Universiti Teknologi MARA Sungai Buloh Campus, Selangor, Malaysia;2. I-PPerFORM, Universiti Teknologi MARA, Malaysia;1. Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran;2. Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran;3. Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran;4. Industrial Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran;5. Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran;6. Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran;1. Department of Radiotherapy, The Second Hospital of Hebei Medical University, Shijiazhuang, 050000, PR China;2. Department of Radiotherapy, The First Affiliated Hospital of Hebei North University, Zhangjiakou, 075000, PR China;3. Department of Oncology, People Hospital Affiliated to Hebei Medical University, Shijiazhuang, 050000, PR China;1. Department of Stem Cells and Regenerative Medicine, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland;2. Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Szczecin, Poland;3. Division of Perinatology and Women’s Diseases, Poznan University of Medical Sciences, Poznan, Poland;4. Clinic of Operational Gynecology, Poznan University of Medical Sciences, Poznan, Poland;5. Department of Pharmacology and Phytochemistry, Institute of Natural Fibers and Medicinal Plants, Poznan, Poland;6. Clinic of Pediatrics, Endocrinology, Diabetology, Metabolic Diseases and Cardiology of the Developmental Age, Pomeranian Medical University, Szczecin, Poland;7. Department of Histocompatibility with Laboratory of Genetic Diagnostics, Regional Blood Center, Poznan, Poland;1. Universidad de Alicante, Departamento de Biotecnología, Alicante, Spain;2. FISABIO - Hospital Universitario de San Juan de Alicante, Servicio de Ginecología y Obstetricia, San Juan de Alicante, Spain;3. Hospital Universitario San Juan de Alicante, Servicio de Ginecología y Obstetricia, San Juan de Alicante, Spain;4. Universidad Miguel Hernández de Elche, Medicina clínica, San Juan de Alicante, Spain;5. Universidad Miguel Hernández de Elche, División de Ginecología, San Juan de Alicante, Spain;6. Cátedra Human Fertility, Universidad de Alicante, Alicante, Spain |
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| Abstract: | Stress can induce reproductive deficits by activating the HPA and causing oxidative stress. Some studies have indicated that the neurologic diseases or disorders induced by stress could be relieved by probiotics. Whether chronic unpredictable stress (CUS)-induced reproductive deficits could be prevented by probiotics is unclear. The present experiment was designed to evaluate the effects of L. rhamnosus Gorbach–Goldin (LGG) on CUS-induced reproductive deficits. Kunming mice were divided into control, stress, and LGG groups randomly. The mice in stress and LGG groups were exposed to CUS for 40days, in the meantime, the mice in LGG group were orally administered with LGG suspension at a dose of 0.3 mL/mouse (1×1010 cells/mL), and the mice in control and stress groups were orally administered with volume-equivalent sterile saline once a day. The results showed that the CUS-induced the sperm deficits including the count, motility, morphology, ultrastructure, DNA integrity, and chromatin condensation were protected by oral administration of LGG. In addition, the change of testosterone level induced by CUS was prevented by up-regulating the expressions of StAR and P450scc in the testes. Moreover, LGG could increase the activities of catalase, glutathione peroxidase, and superoxide dismutase significantly, and decrease the levels of oxidative products malondialdehyde and protein carbonyls significantly, as well as the levels of cyclooxygenase 2, interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, to block the CUS-induced inflammatory response and the oxidative stress. The results indicated that the CUS-induced male reproductive deficits could be prevented by oral administration of LGG. |
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| Keywords: | Stress Reproductive deficit HPA axis Oxidative stress Probiotic |
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