Application of mass spectrometry based proteomics to understand diabetes: A special focus on interactomics |
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| Affiliation: | 1. Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA;2. Center for Diabetes and Metabolic Diseases and the Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN, USA;3. Department of Islet Cell and Regenerative Biology, Joslin Diabetes Center, Department of Medicine, Brigham and Women’s Hospital, and Harvard Stem Cell Institute, Boston, MA, USA;4. ULB Center for Diabetes Research, Medical Faculty, Université Libre de Bruxelles (ULB), Brussels, Belgium;5. Environmental and Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA;6. Computing and Analytics Division, Pacific Northwest National Laboratory, Richland, WA, USA;1. National Centre of Cell Science, Ganeshkhind, Pune, 411007, India;2. Savitribai Phule Pune University, Ganeshkhind, Pune, 411007, India |
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| Abstract: | Diabetes, a multifactorial disorder is characterized by elevated blood glucose levels resulting from changes in lifestyle, genetic and epigenetic changes or aberrations in proteome. In addition, alterations in post-translational modifications (PTMs) and protein-protein interactions (PPIs) also contribute to the development of diabetes pathogenesis. Recent advances in omics technologies have broadened the perspective for systematic investigation of proteome alterations in understanding the pathogenesis of diabetes. Further, PPIs are central to cellular signaling in all living organisms and deranged PPIs lead to diabetic complications. In this context, affinity purification mass spectrometry (AP-MS) along with diverse bioinformatic approaches has proven to be competent in mapping large-scale PPI networks around the critical players in the glucose homeostasis. In this review, we revisit the application of proteomic approaches in investigating proteome alterations and probing PPI networks for a better understanding of the underlying intricacies of the major signaling pathways in altered glucose homeostasis. |
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